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admin
Pridružen/-a: 21.09. 2006, 13:32 Prispevkov: 454
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Gojenje bakterij |
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Kdo od vas goji bakterije, glive ali kaj podobnega. Jaz se bom z tem šele začel ukvarjati in potrebujem nekaj nasvetov :D .
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26 Dec 2006 10:02 |
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Chlorine
moderator
Pridružen/-a: 03.12. 2006, 07:53 Prispevkov: 390
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S tem se ne ukvarjam, ampak potrebuješ nek izoliran prostor, v katerem bojo lahko bakterije uspevale. Ena izmed teh je logično toplejše, mogoče celo vlažnejše okloje.
Bacterial growth is process in which two clone daughter cells are produced by the cell division of one bacterium. Hence, local doubling of the bacterial population occurs. Both daughter cells from the division do not necessarily survive. However, if the number surviving exceeds unity on average, the bacterial population undergoes exponential growth. The measurement of an exponential bacterial growth curve was traditionally a part of the training of all microbiologists.
In autecological studies, bacterial growth can be modeled with four different phases: lag phase (A), exponential or log phase (B), stationary phase (C), and death phase (D).
1. During lag phase, bacteria adapt themselves to growth conditions. It is the period where the individual bacteria are maturing and not yet able to divide.
2. During the exponential phase, the number of new bacteria appearing per unit time is proportional to the present population. This gives rise to the classic exponential growth curve, in which the logarithm of the population density rises linearly with time (see figure). The actual rate of this growth (i.e. the slope of the line in the figure) depends upon the growth conditions, which affect the frequency of cell division events and the probability of both daughter cells surviving. Exponential growth cannot continue indefinitely, however, because the medium is soon depleted of nutrients.
3. During stationary phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the resources that are available to them.
4. At death phase, bacteria run out of nutrients and die.
In reality, these phases are not so well defined, and the curve is much more continuous. Bacterial growth can be suppressed with bacteriostats, without necessarily killing the bacteria. In a synecological, a true-to-nature situation, where more than one bacterial species is present, the growth of microbes is more dynamic and continual.
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26 Dec 2006 10:21 |
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Brom
admin
Pridružen/-a: 21.09. 2006, 13:32 Prispevkov: 454
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Kakšno sredstvo lahko uoprabim za razkuževanje. V mislih sem imel kašen alkohol, potem pa še NaClO?
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26 Dec 2006 12:01 |
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Chlorine
moderator
Pridružen/-a: 03.12. 2006, 07:53 Prispevkov: 390
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Paziti moraš, da jih ne zastrupiš. Se pravi, da raje razkožuj s kakšnimi manj nevarnimi snovmi, raje ne s klorovimi spojinam. Najbolje, da z alkoholom, etra pa ti tudi ne priporočam.
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26 Dec 2006 12:11 |
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Brom
admin
Pridružen/-a: 21.09. 2006, 13:32 Prispevkov: 454
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Ah etra bi mi bilo škoda :D
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26 Dec 2006 13:03 |
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Chlorine
moderator
Pridružen/-a: 03.12. 2006, 07:53 Prispevkov: 390
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Pa za bakterije boš rabu mikroskop, saj boš le tko ugotovu al se ti je namnožilo.
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26 Dec 2006 13:41 |
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Brom
admin
Pridružen/-a: 21.09. 2006, 13:32 Prispevkov: 454
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Sej zato si bom nabavil en za silo mikroskop (v katalogu od lab-teha, stane okoli 28.000sit).
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26 Dec 2006 20:54 |
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roky
admin
Pridružen/-a: 05.12. 2006, 19:16 Prispevkov: 345
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Pa za bakterije boš rabu mikroskop, saj boš le tko ugotovu al se ti je namnožilo. |
ni res če se ti bakterije dobro namnožijo vidiš kolonije s prostim očesom ;)
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26 Dec 2006 20:56 |
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Chlorine
moderator
Pridružen/-a: 03.12. 2006, 07:53 Prispevkov: 390
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Ja ma odvisno kake. Na začetku jih gotovo ne boš mogu gledat, spoh pa če češ videt, ku se množijo je dobro videt pod mikoskopom.
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27 Dec 2006 07:10 |
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kr'en
na pol kemik
Pridružen/-a: 05.12. 2006, 20:53 Prispevkov: 96 Kraj: Domžale |
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Mi smo, ko smo imeli mikrobiologijo v 2 letniku, razkuževali z etanolom(mize), roke pa smo prvo temeljito oprali z rahlo bazičnim milom nato posušili in nazadnje razkužili še z nekim razkužilom ki se ga sigurno da kupiti v sanolaborju, tam tudi zase drugače kupujem razkužilo, da si razkužim roke preden si dam zdravila.
Mi je pa bila MO drugače zelo zanimiva samo profesorice smo se pa bali ko hudič križa in prav zaradi profesorice takrat predmeta nisem bog ve kaj maral.
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30 Dec 2006 09:34 |
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Plantko
experimentira z blagimi kemikalijami
Pridružen/-a: 08.01. 2007, 19:33 Prispevkov: 43 Kraj: blizina Nove Gorice |
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S tem sem se in se leta ukvarjam.Ce te kaj zanima vprasaj na pvt.lp
_________________ nori biokemik |
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10 Jan 2007 12:40 |
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Fischer
na pol kemik
Pridružen/-a: 22.09. 2006, 15:18 Prispevkov: 70 Kraj: Ljubljana |
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Jest sm (biu sm ene 7 let star) gojil bakterije. V lonček sm dal vodo, pa skret papir, travo, narezane šampinjone in govedino in sm za par dni postavu pod omaro v moj sob. Nakar je začelo blazno smrdet in me je mtka dubla pol pa..... sej veste kej je blo :D
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17 Feb 2007 13:19 |
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Brom
admin
Pridružen/-a: 21.09. 2006, 13:32 Prispevkov: 454
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Ojej to pa je res moral bit smrad
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18 Feb 2007 10:24 |
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roky
admin
Pridružen/-a: 05.12. 2006, 19:16 Prispevkov: 345
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omfg .. lepa igra LOL :D
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18 Feb 2007 11:01 |
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Fischer
na pol kemik
Pridružen/-a: 22.09. 2006, 15:18 Prispevkov: 70 Kraj: Ljubljana |
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Smrdel je po mačjemu dreku :D
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18 Feb 2007 17:21 |
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